In the Ghost and Mr. Chicken, the main character, Luther Heggs (Don Knotts) is frightened when the Organ begins playing by itself. The song is the same one that old man Simmons played 20 years earlier to the day after he killed his wife, and right before he committed by flinging himself out out of the organ tower!

The song was written by Vic Mizzy. He is better knows for writing the themes from The Addams Family, The Munsters, Green Acres, and even Spider Man 2.

It appears in the movie three times... and you can find it on the DVD and on the CD, and even on VHS.

Here are the three songs from the movie courtesy of a few YouTube Posters:

The Ghost and Mr. Chicken (1966) Luther's Night In The Old Simmons House

MrKsummer7
http://youtu.be/DA24pLMyvXQ?t=2m59s

The Ghost and Mr Chicken (1966) (13/14) Organ Music Played by the Gardner! FULL MOVIE

CharlieChaplinMovie
http://youtu.be/k8rK-nBtSUY?t=1m28s

The Ghost and Mr Chicken (1966) (14/14) Don Knotts marries his beautiful Bride. FULL MOVIE

CharlieChaplinMovie
http://youtu.be/jird0Kuqez8?t=2m11s

Haunted Organ

Dan Dembicki
http://youtu.be/Nc28EZQHhNw?t=17s

How to play The Ghost and Mr. Chicken Haunted Organ

Skip to 3 minutes, 19 seconds if you want to watch the piece played in it's entirety. HollisKim
http://youtu.be/zVAKRcv6WYo?t=3m19s

The Ghost and Mr. Chicken

HollisKim
http://youtu.be/B8uYXnUcd3A

Creepy Haunted House Organ Music

Skip to 1 minute, 19 seconds for the song.
Adultz94
http://youtu.be/w3C6z8oLhfw?t=1m19s

Ghastly Ghoul at the Organ